High-resolusion histological 3D-imaging: Episcopic Fluorescence Image Capture (EFIC) is widely applied for experimental animals
نویسندگان
چکیده
منابع مشابه
Human cardiac development in the first trimester: a high-resolution magnetic resonance imaging and episcopic fluorescence image capture atlas.
W ith rapid advances in medical imaging, fetal diagnosis of human congenital heart disease is now technically feasible in the first trimester. Although the first human embryological studies were recorded by Hippocrates in 300 to 400 BC, present-day knowledge of normal human cardiac development in the first trimester is still limited. In 1886, 2 articles by Dr His described the development of th...
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Episcopic fluorescence image capturing (EFIC) and high-resolution episcopic microscopy (HREM) are related techniques that are used to generate digital volume data and create three-dimensional (3D) images. Both techniques require specimens that are embedded in an appropriate medium, and images are captured from successive sections before removal from the embedded tissue block. EFIC detects autof...
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This work aims at describing episcopic 3D imaging methods and at discussing how these methods can contribute to researching the genetic mechanisms driving embryogenesis and tissue remodelling, and the genesis of pathologies. Several episcopic 3D imaging methods exist. The most advanced are capable of generating high-resolution volume data (voxel sizes from 0.5x0.5x1 microm upwards) of small to ...
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Episcopic fluorescence image capturing (EFIC) and high-resolution episcopic microscopy (HREM) are related techniques that are used to generate digital volume data and create three-dimensional (3D) images. Both techniques require specimens that are embedded in an appropriate medium, and images are captured from successive sections before removal from the embedded tissue block. EFIC detects autof...
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The creation of highly detailed, three-dimensional (3D) computer models is essential in order to understand the evolution and development of vertebrate embryos, and the pathogenesis of hereditary diseases. A still-increasing number of methods allow for generating digital volume data sets as the basis of virtual 3D computer models. This work aims to provide a brief overview about modern volume d...
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ژورنال
عنوان ژورنال: Congenital Anomalies
سال: 2014
ISSN: 0914-3505
DOI: 10.1111/cga.12057